Heidelberg University Hospital (UKHD) is one of the most important medical centers in Germany and stands for the development of innovative diagnostics and therapies as well as their rapid implementation for the patient. With around 14,000 employees throughout the group in more than 50 clinical departments with more than 2,500 beds, approximately 80,000 patients are treated in full and in part-stationary terms and more than 1,000,000 patients outpatiently. MTLA - Focus on cellular immunotherapy (CAR-T-cell therapy) (m/w/d) at the closest possible time at the Center for Internal Medicine, Department of Hematology, Oncology and Rheumatology in the dynamic and internationally established team of Cellular Immunotherapy.

We are a production plant for new cell therapy products (CAR-T cells, immunomodulatory cells, etc.) with nationwide unique feature. In addition, our focus is on developing new CAR-T cell therapies for acute leukaemia. For a new, third-party funded project, we are looking for qualified, committed and motivated co-authors. Job ID: V000014197 Field of application: Department of Hematology, Oncology and Rheumatology Location: Heidelberg Start date: now Field of activity: Medical-Technical services Type of employment: Full-time (38,5 hours) Duration: limited (first year) Further employment is possible.) Contract: TV-UK

Analytical flow cytometry (FACS) cell culture (isolation and purification of primary cells (predominantly from the blood and bone marrow) of man and mouse, as well as cultivation of primary cells and cell lines; Transduction of different target cells with retroviral vectors;magnetic cell sorting (MACS)) T-cell functionality assays (co-culture, chromium release assay) Molecular biology: PCR techniques (quantitative real-time PCR, RT-PCR, digital PCR), and methods from the field of recombinant DNA (plasmid cloning, sequencing, etc.) gene transfer with MLV-derived retroviral vectors (safety level S2 according to genetic engineering law), and via electroporation Optional collaboration with mouse models for leulemia development and therapy (conversion with mice, animal husbandry, sampling) Cleanroom activities (according to Good Manufacturing Practice, GMP) and the creation of standard treatment instructions (SOPs) Electronic data processing (EDV) and quality management General laboratory tasks and organisation Close cooperation in the friendly, collegial, interdisciplinary team in the fields of medicine, biology and bioinformatics In the case of interest, detailed integration into project planning and analysis beyond the executive work is possible and is supported

Completed training for, for example, biotechnical assistant (BTA) or chemical/technical assistant (CTA) with state recognition or comparable knowledge and skills or completed vocational training medical-technical assistant (MTAF), medical-technical/laboratory assistant (MTLA or MTA-L), or veterinary-technical/technical assistant (VMTA) Experiences in molecular biology (e.g. plasmid cloning, PCR) desirable skills in handling primary cells / different cell cultures desired Experience in working with gene transfer vectors would be advantageous Expertise and experience in flow cytometry would be advantageous Experiences with work under GMP conditions and/or quality management are advantageous Knowledge in handling electronic data processing (Microsoft Office package) Ideally good English skills Commitment, independent experimental work, team and communication skills, flexibility, reliability, readiness to use, organisational capacity

Interesting workplace, esteeming interaction and open knowledge sharing in the team Structured integration concept Internal continuing and continuing training Parttime employment is possible in principle collective agreement remuneration, attractive occupational pension schemes 30 days off Continued: Jobticket (Germanyticket) Family-friendly work environment: cooperation on childcare, child-care allowance, counselling for employees with dependent family members Various health, prevention and sports offers Note: UKHD is subject to the provisions of the Infection Protection Act. Therefore, a valid measles immunity certificate is necessary for all persons employed in the UKHD.

The UKHD lives diversity and appreciates diversity.

Regardless of age, gender, sexual identity, disability, origin or religion, we offer all the same opportunities. If a gender is underrepresented in a specific area, we attach particular importance to counteract this imbalance. In the case of equal suitability, competence and professional performance, we give priority to people with severe disabilities when considering open positions. JOBV1_EN