PhD position (m/f/d) Department of Medicine V, Hematology, Oncology, Rheumatology

Branche: Gesundheits- und Krankenpfl... Branche: Gesundheits- und Krankenpflege, Rettungsdienst und Geburtshilfe

The University Hospital Heidelberg is one of the major healthcare centers in Germany. Our objective is the development of innovative diagnostics and therapies as well as their quick implementation for the patient. With about 14,000 employees in more than 50 specialized clinical departments with almost 2,500 beds, about 80,000 patients in part-time and full-time inpatient treatment as well as 1,000,000 patients in ambulant treatment are medicated each year.

PhD position (m/f/d) Multimodal approaches to understand and overcome niche-related adaptive drug resistance in FLT3-mutated AML

wanted for the next possible date at the Department of Medicine V, Hematology, Oncology, Rheumatology

For our project “Multi-modal proteomics to understand and overcome niche-related adaptive drug-resistance mechanisms in FLT3-mutated AML“ we are looking for a PhD student. The project is is part of the SFB/CRC 1709 “Cellular Plasticity in Myeloid Malignancies: From Mechanisms to Therapies-. In this CRC we will focus on myeloid malignancies as a model to dissect the various molecular mechanisms that enable and regulate cancer cell plasticity in AML. Our overall goal is to pave the way for new cancer therapies.

In our project we want to decipher the niche-related adaptive molecular mechanisms that underlie and promote the emergence of FLT3-inhibitor resistant clones. The aim of this PhD position is to investigate primary cells, PDX and cell line models, and use a variety of methods to specifically identify adaptive changes in untreated, treated and drug-resistant cell populations, as well as niche-related phenotypic alterations upon drug resistance and their underlying mechanisms and pathways. These methods will include various assays to assess for intercellular interaction and migration, CRISPR-based perturbation-of-adhesion and synthetic lethality screening, drug synergy assays, and others. The PhD student will work closely together with a PhD student in the lab of Prof. Jeroen Krijgsveld, which will focus on the proteomics part of the joint SFB/CRC project applying state-of-the-art proteomic approaches, e.g. low-input/single-cell proteomics, nascent proteomics, phosphoproteomics, etc. Furthermore, for PDX/in-vivo experiments we have an established collaboration within the CRC.

• Job-ID: V000014485
• Field of application: Klinik für Hämatologie, Onkologie und Rheumatologie
• Location: Heidelberg
• Job Category: Science and teaching
• Working hours: Part time (65 %)
• Limitation: Temporary (2 years with option of extension)
• Contract: TV-L